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Catalog Number | orb248486 |
---|---|
Category | Antibodies |
Description | This antibody recognizes a 53kDa protein, identified as the Ornithine Decarboxylase. ODC-1 is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC-1 mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. Activity in colorectal carcinomas is greater than those in adenomas and normal mucosa. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | ODC1/485 |
Tested applications | FACS, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Mouse IgG1, kappa |
Immunogen | Recombinant human ODC-1 protein was used as the immunogen for this antibody. |
Dilution range | Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT (1) (2) |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This ODC-1 antibody is available for research use only. |
Entrez | 4953 |
Storage | Store the ODC-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human prostate carcinoma tissue using ODC-1 antibody
Western blot analysis of human samples using ODC-1 antibody
FACS, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
IHC-P, WB | |
Human | |
Mouse | |
Recombinant | |
Unconjugated |
IHC-P, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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