Nrf2 rabbit pAb Antibody

SKU: orb765860

Description

Rabbit polyclonal antibody to Nrf2

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Nrf2. AA range:556-605
• Molecular Weight: 75-100
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-NFE2L2 antibody, anti-NRF2 antibody, anti-Nuclear factor erythroid 2-related factor 2 antibody, anti-NF-E2-related factor 2 antibody, anti-NFE2-related factor 2 antibody, anti-HEBP1 antibody, anti-Nuclear factor antibody, anti- erythroid derived 2 antibody, anti-like 2 antibody

Western blot analysis of lysates from 1) Hela cell, 2) Hela cells knockdown by NRF2-siRNA (F:CAUUGAUGUUUCUGAUCUATT, R:UAGAUCAGAAACAUCAAUGTT), (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody was diluted at 1:10000, 37°C 1 hour. (Red) GAPDH Monoclonal Antibody (5B7) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody was diluted at 1:10000, 37°C 1 hour.