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NFAT5 (phospho Ser1197) rabbit pAb
Description
Images & Validation
−| Tested Applications | ELISA, IF |
|---|---|
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Reactivity | Human, Mouse |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NFAT5 around the phosphorylation site of Ser1197. AA range:1171-1220 |
| Molecular Weight | 166kD |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NFAT5 (Phospho-Ser1197) Antibody.

Immunofluorescence analysis of COS7 cells, using NFAT5 (Phospho-Ser1197) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Quick Database Links
Protocol Information
NFAT5 (phospho Ser1197) rabbit pAb (orb767415)
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