MerTK/Tyro3 (phospho Tyr749/681) rabbit pAb

• Catalog Number: orb767352
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MerTK/Tyro3 (phospho-Tyr749/681)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human MER/SKY around the phosphorylation site of Tyr749/681. AA range:716-765
• MW: 97
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-MERTK antibody, anti-MER antibody, anti-Tyros
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of 3T3 cells using Phospho-MerTK/Tyro3 (Y749/681) Polyclonal Antibody diluted at 1:500.

Western Blot analysis of 293T/mouse-heart using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.

Immunohistochemistry analysis of paraffin-embedded human brain, using MER/SKY (Phospho-Tyr749/681) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 5', using MER/SKY (Phospho-Tyr749/681) Antibody. The lane on the right is blocked with the phospho peptide.