MEK-1/2 (phospho Ser222/226) rabbit pAb

SKU: orb769700

Description

Rabbit polyclonal antibody to MEK-1/2 (phospho-Ser222/226)

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human MEK1/2 around the phosphorylation site of Ser221. AA range:193-242
• Molecular Weight: 44
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-MAP2K1 antibody, anti-MEK1 antibody, anti-PRKMK1 antibody, anti-Dual specificity mitogen-activated protein kinase kinase 1 antibody, anti-MAP kinase kinase 1 antibody, anti-MAPKK 1 antibody, anti-MKK1 antibody, anti-ERK activator kinase 1 antibody, anti-MAPK/ERK kinase 1 antibody, anti-MEK 1 antibody, anti-MAP2K2 antibody, anti-MEK2 antibody, anti-MKK2 antibody, anti-PRKMK2 antibody, anti-Dual antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK1/2 (Phospho-Ser221) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from K562 cells treated with serum 20% 15' and Jurkat cells treated with EGF, using MEK1/2 (Phospho-Ser221) Antibody. The lane on the right is blocked with the phospho peptide.