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MDM2 (phospho Ser166) rabbit pAb

Catalog Number: orb769084

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb769084
CategoryAntibodies
DescriptionRabbit polyclonal antibody to MDM2 (phospho-Ser166)
ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Monkey, Mouse
Concentration1 mg/ml
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
ImmunogenThe antiserum was produced against synthesized peptide derived from human MDM2 around the phosphorylation site of Ser166. AA range:132-181
MW90
Tested applicationsELISA, IF, IHC-P, WB
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesAnti-MDM2 antibody, anti-E3 ubiquitin-protein liga
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NoteFor research use only
MDM2 (phospho Ser166) rabbit pAb

Immunofluorescence analysis of Hela cell. 1, MDM2 (phospho Ser166) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HAO1 Monoclonal Antibody (Mix) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

MDM2 (phospho Ser166) rabbit pAb

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MDM2 (Phospho-Ser166) Antibody.

MDM2 (phospho Ser166) rabbit pAb

Immunofluorescence analysis of A549 cells, using MDM2 (Phospho-Ser166) Antibody. The picture on the right is blocked with the phospho peptide.

MDM2 (phospho Ser166) rabbit pAb

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MDM2 (Phospho-Ser166) Antibody. The picture on the right is blocked with the phospho peptide.