Mdm2 Antibody (C-term)

SKU: orb1937377

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cell Biology, Infectious Disease & Virology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:10~50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB00757
• Target: This Mdm2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-424 amino acids from the C-terminal region of human Mdm2.
• Molecular Weight: 55233 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

E3 ubiquitin-protein ligase Mdm2, 632-, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2, MDM2

All lanes: Anti-MDM2 Antibody (C-term) at 1:1000 dilution. Lane 1: CCRF-CEM whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: A549 whole cell lysate. Lane 4: Daudi whole cell lysate. Lane 5: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Mdm2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Mdm2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

This Mdm2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-424 amino acids from the C-terminal region of human Mdm2.

UniProt

Q00987

RefSeq (Protein):NP_002383.2, NP_001138811.1, NP_001265391.1