MARCH5 rabbit pAb

SKU: orb769548

Description

Rabbit polyclonal antibody to MARCH5

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution range: WB 1:500-2000 Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human MARCH5. AA range:21-70
• Molecular Weight: 37
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-MARCH5 antibody, anti-RNF153 antibody, anti-E3 ubiquitin-protein ligase MARCH5 antibody, anti-Membrane-associated RING finger protein 5 antibody, anti-Membrane-associated RING-CH protein V antibody, anti-MARCH-V antibody, anti-Mitochondrial ubiquitin ligase antibody, anti-MITOL antibody, anti-RING finger protei antibody

Immunofluorescence analysis of Hela cell. 1, MARCH5 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Immunofluorescence analysis of A549 cells, using MARCH5 Antibody. The picture on the right is blocked with the synthesized peptide.