MAPKAPK-2 (phospho Thr222) rabbit pAb

• Catalog Number: orb770879
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MAPKAPK-2 (phospho-Thr222)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human MAPKAPK-2 around the phosphorylation site of Thr222. AA range:188-237
• MW: 45
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-MAPKAPK2 antibody, anti-MAP kinase-activated
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MAPKAPK-2 (Phospho-Thr222) Antibody.

Immunohistochemistry analysis of paraffin-embedded human heart, using MAPKAPK-2 (Phospho-Thr222) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells treated with UV 15', using MAPKAPK-2 (Phospho-Thr222) Antibody. The lane on the right is blocked with the phospho peptide.