Lfc (phospho Ser886) rabbit pAb Antibody

SKU: orb770855

Description

Rabbit polyclonal antibody to Lfc (phospho-Ser885)

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Rho/Rac Guanine Nucleotide Exchange Factor 2 around the phosphorylation site of Ser885. AA range:851-900
• Molecular Weight: 111
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-ARHGEF2 antibody, anti-KIAA0651 antibody, anti-LFP40 antibody, anti-Rho guanine nucleotide exchange factor 2 antibody, anti-Guanine nucleotide exchange factor H1 antibody, anti-GEF-H1 antibody, anti-Microtubule-regulated Rho-GEF antibody, anti-Proliferating cell nucleolar antigen p40 antibody

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Rho/Rac Guanine Nucleotide Exchange Factor 2 (Phospho-Ser885) Antibody.

Western blot analysis of lysates from HeLa cells treated with TSA 400nM 24H and Jurkat cells treated with forskolin 40nM 30', using Rho/Rac Guanine Nucleotide Exchange Factor 2 (Phospho-Ser885) Antibody. The lane on the right is blocked with the phospho peptide.

Western Blot analysis of 1 A431 treated with LPS, 2 A431, using primary antibody at 1:1000 dilution. Secondary antibody was diluted at 1:10000.