Lamin B1 rabbit pAb

• Catalog Number: orb766785
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Lamin B1
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human LMNB1. AA range:391-440
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 70
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-LMNB1 antibody, anti-LMN2 antibody, anti-LMNB
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from 1) Hela cell, 2) Hela cells knockdown by siRNA (F:GCUUCUUGAUGUAAAGUUATT, R:UAACUUUACAUCAAGAAGCTT), (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody was diluted at 1:10000, 37°C 1 hour. (Red) actin Monoclonal Antibody antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody was diluted at 1:10000, 37°C 1 hour.