Cytokeratin 19 Antibody

SKU: orb248454

Description

This antibody is specific to the rod domain of human Cytokeratin 19 (CK19), a polypeptide of 40kDa. Its epitope maps between amino acid 312-335. Cytokeratin 19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Cytokeratin 19 antibody reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Cytokeratin 19 antibody is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cytokeratin 19 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, lambda
• Clone No.: A53-B/A2.26
• Immunogen: Human breast cancer MCF-7 cells were used as the immunogen for this Cytokeratin 19 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Disclaimer: For research use only

Western blot testing of human 1) HepG2 and 2) MCF7 cell lysate with Cytokeratin 19 antibody (clone A53-B/A2.26). Predicted molecular weight ~43 kDa.

Western blot testing of human PC3 cell lysate using of Cytokeratin 19 antibody (clone A53-B/A2.26). Predicted molecular weight ~43 kDa.

IHC testing of FFPE human colon carcinoma stained with Cytokeratin 19 antibody (clone A53-B/A2.26).

Immunofluorescent staining of MeOH fixed human MCF7 cells with Cytokeratin 19 antibody (clone A53-B/A2.26, blue) and Reddot nuclear stain (red).

SDS-PAGE analysis of purified, BSA-free Cytokeratin 19 antibody (clone A53-B/A2.26) as confirmation of integrity and purity.