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| Catalog Number | orb768845 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to JAK2 (phospho-Tyr221) |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human JAK2 around the phosphorylation site of Tyr221. AA range:191-240 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 136 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-JAK2 antibody, anti-Tyrosine-protein kinase J Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using JAK2 (Phospho-Tyr221) Antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using JAK2 (Phospho-Tyr221) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from K562 cells, using JAK2 (Phospho-Tyr221) Antibody. The lane on the right is blocked with the phospho peptide.
Phospho-JAK2 (Tyr221) Rabbit pAb [orb2711200]
ELISA, FC, ICC, IF, IHC-Fr, IHC-P
Human, Zebrafish
Polyclonal
Unconjugated
100 μl, 50 μl
