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ITGB4
Description
Images & Validation
−| Tested Applications | ELISA, FC, IHC |
|---|---|
| Dilution range | IHC: 1/200 - 1/1000; RCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 10B10D5 |
| Immunogen | Purified recombinant fragment of human ITGB4 (AA: 1619-1822) expressed in E. Coli. |
| Molecular Weight | 202kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Ascitic fluid containing 0.03% sodium azide. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 115.4&81.6 KDa. Observed: (75-95)&(105-135) KDa
1 mg, 500 μg, 50 μg, 10 μg

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Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of ITGB4 antibody

Flow cytometric analysis of A549 cells lysates using ITGB4 antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded cervical cancer tissues using ITGB4 antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded esophageal cancer tissues using ITGB4 antibody

Western blot analysis of human ITGB4 using ITGB4 antibody

Western blot analysis of A549 (Lane 1), A431 (Lane 2), MCF-7 (Lane 3) SW620 (Lane 4) cell lysate using ITGB4 antibody
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Protocol Information
ITGB4 (orb197853)
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