IRS-1 (phospho Ser312) rabbit pAb

SKU: orb764325

Description

Rabbit polyclonal antibody to IRS-1 (phospho-Ser312)

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution range: WB 1:500-2000;IHC-p 1:50-300
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser312. AA range:279-328
• Molecular Weight: 170
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-IRS1 antibody, anti-Insulin receptor substrate 1 antibody, anti-IRS-1 antibody

Western Blot analysis of various cells using Phospho-IRS-1 (S312) Polyclonal Antibody diluted at 1:1000.

Western blot analysis of 293T Hela mouse-spleen lysis using Phospho-IRS-1 (S312) antibody. Antibody was diluted at 1:1000.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IRS-1 (Phospho-Ser312) Antibody.

Immunofluorescence analysis of HeLa cells, using IRS-1 (Phospho-Ser312) Antibody. The picture on the right is blocked with the phospho peptide.