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Catalog Number | orb764213 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Integrin beta1 (phospho-Thr788) |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Concentration | 1 mg/ml |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Immunogen | The antiserum was produced against synthesized peptide derived from human Integrin beta1 around the phosphorylation site of Thr788. AA range:749-798 |
MW | 135 |
Tested applications | ELISA, IHC-P, WB |
Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Anti-ITGB1 antibody, anti-FNRB antibody, anti-MDF2 Read more... |
Note | For research use only |
Western blot analysis of lysates from 1) Hela, 2) 3T3, 3) Jurkat cells, was diluted at 1:10000, 37° 1hour. antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody was diluted at 1:10000, 37° 1hour.
Western Blot analysis of various cells using Phospho-Integrin β1 (T788) Polyclonal Antibody.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Integrin beta1 (Phospho-Thr788) Antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Integrin beta1 (Phospho-Thr788) Antibody. The picture on the right is blocked with the phospho peptide.