ZNF148 Antibody

SKU: orb1256668

Description

ZNF148 Antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:20 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 690-794 of human ZNF148 (NP_068799.2).
• Target: ZNF148
• Molecular Weight: Observed: 105kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Zinc finger protein 148, Transcription factor ZBP-89, Zinc finger DNA-binding protein 89, ZNF148, ZBP89

Western blot analysis of extracts of various cell lines, using ZNF148 Antibody (orb1256668) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat lung using ZNF148 antibody (orb1256668) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat liver using ZNF148 antibody (orb1256668) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using ZNF148 antibody (orb1256668) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using ZNF148 antibody (orb1256668) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung using ZNF148 antibody (orb1256668) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using ZNF148 antibody (orb1256668) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human appendix using ZNF148 antibody (orb1256668) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human smooth muscle using ZNF148 antibody (orb1256668) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using ZNF148 antibody (orb1256668) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using ZNF148 antibody (orb1256668) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using ZNF148 antibody (orb1256668) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using ZNF148 antibody (orb1256668) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A-549 cells using ZNF148 antibody (orb1256668).

Quick Database Links

Gene Symbol

ZNF148

UniProt

Q9UQR1