VEGF Antibody

• Catalog Number: orb1294346
• Category: Antibodies
• Description: Rabbit polyclonal antibody to VEGF
• Target: VEGF
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit-IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide / encompassing a sequence within the center region
• Tested applications: IF, IHC, WB
• Dilution range: Western Blot 1:500–1:1000 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:100–1:300 Immunoprecipitation : Reported in the literature, not verified in house.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Cardiovascular Research
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294346 anti-VEGF antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling VEGF with orb1294346 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling VEGF with orb1294346 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Kidney cancer tissue labeling VEGF with orb1294346 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Liver and Testis cancer tissue labeling VEGF with orb1294346 at 1/100.

Immunohistochemical analysis of paraffin embedded mouse colon labeling VEGF with orb1294346 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse Lung labeling VEGF with orb1294346 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling VEGF with orb1294346 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling VEGF with orb1294346 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling VEGF with orb1294346 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling VEGF with orb1294346 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling VEGF with orb1294346 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

VEGF antibody at 1/1000 dilution. Lysates/proteins at 35 µg per lane. This blot was produced using a 15% SDS-PAGE.

VEGF antibody at 1/1000 dilution. Lysates/proteins at 35 µg per lane. This blot was produced using a 15% SDS-PAGE.