TYRP1 Rabbit Polyclonal Antibody

SKU: orb158651

Description

TYRP1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Equine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TYRP1 (101-200/537aa)
• Target: TYRP1
• Molecular Weight: 58 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

TRP; CAS2; CATB; GP75; OCA3; TRP1; TYRP; TRP-1; b-PROTEIN; tyrosinase related protein 1; TYRRP; 5, 6-dihydroxyindole-2-carboxylic acid oxidase; EC:1.14.18; Catalase B; Glycoprotein 75; Melanoma antigen gp75; Tyrosinase-related protein 1; TYRP1_HUMAN;

Paraformaldehyde-fixed, paraffin embedded (Human skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Tyrosinase-related protein 1) Polyclonal Antibody, Unconjugated (orb158651) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Sample: B16 (Mouse) cell Lysate at 40 ug, Primary: Anti-TRP1 (orb158651) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 55 kD.

Quick Database Links

Gene Symbol

TYRP1

UniProt

P17643