SOX2 Rabbit Polyclonal Antibody

SKU: orb500791

Description

SOX2 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SOX2 (227-317/317aa)
• Target: SOX2
• Molecular Weight: 34 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

transcriptional factor SOX2; ANOP3; cb236; delta EF2a; lcc; MCOPS3; MGC148683; MGC2413; RGD1565646; Sex determining region Y box 2; Sex determining region Y-box 2; SOX 2; SRY(sex determining region Y) box 2; SRY box containing gene 2; SRY related HMG box 2; SRY related HMG box gene 2; SRY-box 2; ysb; SOX2_HUMAN; Transcription factor SOX-2; SOX2_HUMAN.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-SOX2 antibody (orb500791), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: U251. Primary Antibody (green line): Rabbit Anti-SOX2 antibody (orb500791), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Cell: NCCIT, Concentration: 1:100, Host/Isotype: Rabbit/IgG, Flow cytometric analysis of Rabbit IgG isotype control (orb500791) on NCCIT (green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody.

Paraformaldehyde-fixed, paraffin embedded (Mouse embryo), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SOX2) Polyclonal Antibody, Unconjugated (orb500791) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-SOX2 (orb500791) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD.

Sample: Eye (Mouse) Lysate at 40 ug, Primary: Anti-SOX2 (orb500791) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD.

Sample: Eye (Rat) Lysate at 40 ug, Primary: Anti-SOX2 (orb500791) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD.

Quick Database Links

Gene Symbol

SOX2

UniProt

P48431