SARS-CoV-2 Spike RBD Neutralizing Antibody, monoclonal, Chimeric

• Catalog Number: orb1274213
• Category: Antibodies
• Description: SARS-CoV-2 Spike RBD Neutralizing Antibody, monoclonal, Chimeric
• Target: S
• Clonality: Recombinant
• Isotype: Human IgG1/kappa
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Buffer/Preservatives: Lyophilized from 0.22 um filtered solution in PBS, pH 7.4 . Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.
• Purification: >95% as determined by SDS-PAGE.
• Immunogen: Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified recombinant SARS-CoV-2 Spike S1 Protein. Pseudovirus assay shows that this antibody has potent neutralizing activity against pseudovirus bearing SARS-CoV-2 Spike protein.
• UniProt ID: P0DTC2
• Tested applications: ELISA, WB
• Application notes: This antibody can be paired with other Anti-SARS-CoV-2 Spike S1 antibodies to detect SARS-CoV-2 Spike S1 protein in sandwich ELISA or LFA assay.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: spike glycoprotein
• Research Area: Infectious Disease & Virology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Immobilized SARS-CoV-2 S1 protein, His Tag at 2 ug/mL (100 uL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 with a linear range of 0.2-3 ng/mL (QC tested).

Detection SARS-CoV-2 S protein RBD by Sandwich ELISA Assay. Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 at 2 ug/mL (100 uL/well) can bind S protein RBD. And then add Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 at 1:5000. Detection was performed using HRP-conjugated streptavidin with sensitivity of 25 pg/mL (Routinely tested).

Immobilized Human ACE2, His Tag at 2 ug/mL (100 uL/well) can bind pre-mixed increasing concentrations of Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 and 0.4 ug/mL (50 uL/well) Biotinylated S protein RBD, His,Avitag with a half maximal inhibitory concentration (IC50) of 0.33 ug/mL (Routinely tested).

Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD, His Tag with an affinity constant of 0.375 nM as determined in a SPR assay (Biacore T200) (Routinely tested).