SARS-CoV-2 RBD Potent Neutralizing Antibody, monoclonal, Chimeric

• Catalog Number: orb1274220
• Category: Antibodies
• Description: SARS-CoV-2 RBD Potent Neutralizing Antibody, monoclonal, Chimeric
• Target: S
• Clonality: Recombinant
• Isotype: Human IgG1/kappa
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Buffer/Preservatives: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 . Normally trehalose is added as protectant before lyophilization.
• Purification: >95% as determined by SDS-PAGE.
• Immunogen: Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM128) is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. Pseudovirus assay shows that this antibody has potent neutralizing activity against pseudovirus bearing SARS-CoV-2 Spike protein.
• UniProt ID: P0DTC2
• Tested applications: ELISA, WB
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: spike glycoprotein
• Research Area: Infectious Disease & Virology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Immobilized SARS-CoV-2 S protein RBD, His Tag at 1 ug/mL (100 uL/well) can bind Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM128) with a linear range of 0.05-3.13 ng/mL (QC tested).

Serial dilutions of Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 was detected by SARS-CoV-2 Inhibitor screening Kit with a half maximal inhibitory concentration (IC50) of 1.197 ug/mL (Routinely tested).

Serial dilutions of Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody was incubated with SARS-CoV-2 pseudotyped virus at 37C for 1 hour. Afterward, Huh-7 cells were added, followed by incubation at 37C for 24h. Chemiluminescence detection was performed and the virus neutralization titers (IC50) is 0.03255 nM using the Reed-Muench method.

Loaded Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 on AHC Biosensor, can bind SARS-CoV-2 S1 protein, His Tag with an affinity constant of 4.42 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).