SARS-CoV-2 (COVID-19) Spike RBD + SD1 Recombinant Protein

SKU: orb1231587

Description

SARS-CoV-2 (COVID-19) Spike RBD + SD1 Recombinant Protein

Images & Validation

• Tested Applications: ELISA, WB

Key Properties

• Source: HEK293 cells
• Tag: His tag at C-terminus
• Molecular Weight: 31.8 kD
• Protein Sequence: Arg319-Ser591
• Purity: < 95%

Storage & Handling

• Storage: The protein is stable in a liquid state at -70°C for 12 months. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS
• Concentration: 1 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

SARS-CoV-2 S, coronavirus spike Protein, cov spike Protein, ncov RBD Protein, ncov s1 Protein, ncov s2 Protein, novel coronavirus spike Protein, RBD Protein, S1 Protein, S2 Protein, Spike RBD Protein

Binding Assay of ACE2 and SARS-CoV-2 (COVID-19) Spike RBD + SD1 Recombinant Protein, Coating: SARS-CoV-2 (COVID-19) Spike RBD + SD1 recombinant protein (orb1231587) from 0.3 ng to 1000 ng. Capture: ACE2 recombinant protein, orb1231604 (0.5 μg/mL). Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution.

SDS-PAGE Validation with SARS-CoV-2 (COVID-19) Spike RBD + SD1 Recombinant Protein. Loading: 1 μg SARS-CoV-2 (COVID-19) Spike RBD + SD1 recombinant protein, orb1231587. Observed: 40kD

Western Blot Validation with SARS-CoV-2 (COVID-19) Spike RBD + SD1 Recombinant Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike RBD + SD1 recombinant protein, orb1231587. Antibodies: SARS-CoV-2 (COVID-19) Spike RBD, orb1239994, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 μg/mL and Lane 2: 2 μg/mL.

ELISA Validation of SARS-CoV-2 (COVID-19) Spike RBD + SD1 Recombinant Protein. Coating Antigen: SARS-CoV-2 (COVID-19) Spike RBD + SD1 recombinant protein (orb1231587) from 0.3 ng to 1000 ng. Capture Antibody: SARS-CoV-2 (COVID-19) Spike RBD antibody, orb1239994 (1 μg/mL). Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution.