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Item 1 of 6
SARS-CoV-2 (COVID-19) S1 Protein, mFc Tag
Catalog Number: orb640170
| Catalog Number | orb640170 |
|---|---|
| Category | Proteins |
| Description | It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
| Reactivity | Virus |
| Tag | C-mFc(mIgG2a) |
| Form/Appearance | Powder |
| Purity | 90% |
| Conjugation | Unconjugated |
| MW | 101.9 kDa |
| Target | Spike S1 |
| Protein Sequence | QHD43416.1 |
| Source | SARS-CoV-2 S1 protein, Mouse IgG2a Fc Tag (orb640170) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1). |
| Expression System | HEK293 |
| Biological Origin | SARS-CoV-2 |
| Expression Region | Val 16 - Arg 685 |
| Endotoxins | 1.0 EU per μg |
| NCBI | QHD43416.1 |
| Storage | -20°C |
| Buffer/Preservatives | PBS, pH7.4 |
| Alternative names | Spike,S1 protein,Spike glycoprotein Subunit1,S gly Read more... |
| Note | For research use only |
| Application notes | This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 101.9 kDa. The protein migrates as 115-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expiration Date | 6 months from date of receipt. |
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of of SARS-CoV-2 (COVID-19) S1 protein.
SDS-page analysis of SARS-CoV-2 (COVID-19) S1 protein.
Flow cytometric analysis of Vero E6 cells using SARS-CoV-2 (COVID-19) S1 protein.
Flow cytometric analysis of Vero E6 cells using SARS-CoV-2 (COVID-19) S1 protein.
Flow cytometric analysis of Vero E6 cells using SARS-CoV-2 (COVID-19) S1 protein.
SARS-CoV-2 (COVID-19) S1 protein.
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SARS-CoV-2 (2019-nCoV) S1 protein NTD, mFc Tag [orb689471]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 59.1 kDa after removal of the signal peptide.The apparent molecular mass of S1-NTD-mFc is approximately 70-100 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg 
SARS-CoV-2 (COVID-19) S Protein RBD, mFc Tag [orb640173]
Unconjugated
95%
52.0 kDa
SARS-CoV-2 S protein RBD, Mouse IgG2a Fc Tag (orb640173) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541 (Accession # QHD43416.1).
100 μg, 1 mgRecombinant COVID-19/SARS-CoV-2 Spike RBD protein, mFc Tag [orb1141464]
ELISA, WB
≥95% as determined by SDS-PAGE
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (RBD) (YP_009724390.1) was fused a mFc tag at N terminus was expressed from Mammalian cells can bind with ACE2.
100 μg, 1 mg
