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| Catalog Number | orb640173 |
|---|---|
| Category | Proteins |
| Description | It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
| Target | Spike RBD |
| Conjugation | Unconjugated |
| Tag | C-mFc(mIgG2a) |
| Reactivity | Virus |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| UniProt ID | QHD43416.1 |
| MW | 52.0 kDa |
| Application notes | This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 52.0 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Expression Region | Arg 319 - Phe 541 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Research Area | Infectious Disease & Virology |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 52.2 kDa after removal of the signal peptide. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 51.3 kDa after removal of the signal peptide. | |
Mammalian |
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