Phospho-LC3C(S12) Antibody

• Catalog Number: orb1931205
• Category: Antibodies
• Description: Phospho-LC3C(S12) Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9H492
• MW: 14272 Da
• Tested applications: DOT, WB
• Dilution range: WB - 1:1000, DB - 1:500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Research Area: Cancer Biology, Cardiovascular Research, Cell Biol
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Dot blot analysis of Phospho-LC3 (APG8a) - S12 Antibody and Nonphospho-LC3 (APG8a) Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Something like SH-SY5Y cells expressing GFP-LC3-WT or-S12D treated with rapamycin or vehicle for 1 h.

Immunoblots of SH-SY5Y cells treated with MPP+ for 24h. The data shows that treatment with MPP+ showed no significant change in level of LC3.

Immunoblots of SH-SY5Y cells treated with rapamycin for 1 h. The data shows that treatment with rapamycin showed no significant change in level of LC3.

Immunoblots of phosphorylated LC3 (phospho-LC3) in CHO cell culture. LC3 and LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with anti-phospho-LC3 S12 antibody. LC3 = microtubule-associated protein light chain-3; S12A = replacement of the amino acid position 12 serine of LC3 with alanine. WT = wildtype LC3-transfected cell lysates; S12A = LC3 S12A mutant-transfected cell lysates; Empty vector = vector with no LC3 gene. Molecular size: LC3-I = 16kDa, and LC3-II = 14 kDa