Phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) Rabbit Polyclonal Antibody (BF647)

• Catalog Number: orb1975858
• Category: Antibodies
• Description: Phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) Rabbit Polyclonal Antibody (BF647)
• Target: GSK3B
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: BF647
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit, Sheep
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Tyr216 VS(p-Y)IC
• Tested applications: FC
• Dilution range: Flow-Cyt=1μg /test
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: GSK3B(phospho-Tyr216); GSK3B(phospho-Y216); p-GSK-
• Research Area: Cancer Research, Cardiovascular Research, Cell Bio
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) antibody (orb1975858), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-AF647. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells then were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.