Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody (BF647)

• Catalog Number: orb1587525
• Category: Antibodies
• Description: Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody (BF647)
• Target: PRKAA2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: BF647
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human AMPK alpha 2 around the phosphorylation site of Thr172 LR(p-T)SC
• Tested applications: FC, IF
• Dilution range: IF=1:100-500, Flow-Cyt=1ug/test
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: AMPK alpha 1(phospho T183)+AMPK alpha 2(phospho T1
• Research Area: Cell Biology, Energy Metabolism, Fatty Acid Oxidat
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Rat spleen. Primary Antibody (green line): Rabbit Anti-phospho-AMPK alpha-2 (Thr172) antibody (orb1587525), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.