Nibrin rabbit pAb

• Catalog Number: orb769211
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Nibrin
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Nibrin. AA range:251-300
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 85
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-NBN antibody, anti-NBS antibody, anti-NBS1 an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of A549 cells, using Nibrin Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat cells, using Nibrin Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from K562 cells using Nibrin antibody.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).