NBN Antibody (C-term)

• Catalog Number: orb1937963
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB19072
• Tested applications: FC, IF, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 84959 Da
• Target: This NBN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 602-630 amino acids from the C-terminal region of human NBN.
• UniProt ID: O60934
• NCBI: NP_002476.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Nibrin, Cell cycle regulatory protein p95, Nijmege
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Confocal immunofluorescent analysis of NBN Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

NBN Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

NBN Antibody (C-term) western blot analysis in NCI-H460, NCI-H292 cell line lysates (35 ug/lane). This demonstrates the NBN antibody detected the NBN protein (arrow).