Mouse IgG (H&L) Antibody Rhodamine Conjugated

• Catalog Number: orb347358
• Category: Antibodies
• Description: Mouse IgG (H&L) antibody (TRITC)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: TRITC
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.
• Immunogen: Mouse IgG whole molecule
• Tested applications: DOT, FC, FLISA, IF, WB
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Application notes: Anti-Mouse IgG Rhodamine Antibody has been tested by dot blot and western blot and is available in a variety of formats. Anti IgG secondary antibody conjugated is suitable for ELISA, Immunohistochemistry western blotting as well as other peroxidase antibody based assays.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: goat anti-Mouse IgG rhodamine conjugated Antibody,
• Note: For research use only

Dot Blot of Rhodamine Conjugated Goat-a-Mouse IgG. Antigen: Mouse IgG. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng Primary antibody: none Secondary antibody: Rhodamine Conjugated Goat-a-Mouse IgG secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 60 min at RT.

Western Blot of Rhodamine Conjugated Goat anti-Mouse IgG Secondary Antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Rhodamine goat secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): none.