Mouse IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

• Catalog Number: orb347568
• Category: Antibodies
• Description: Mouse IgG (H&L) antibody (Alkaline Phosphatase)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: AP
• Reactivity: Mouse
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose followed by solid phase adsorption to remove unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reactivity was observed against Human serum proteins.
• Immunogen: Mouse IgG whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:9,800, IHC: 1:100 - 1:500, WB: 1:500 - 1:2,000
• Application notes: Anti-Mouse IgG Alkaline Phosphatase conjugate has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Alternative names: rabbit anti-Mouse IgG Antibody alkaline phosphatas
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot of Alkaline Phosphatase Conjugated Rabbit anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase mouse secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min RT. Predicted/Observed size: 55 kDa, 28 kDa for Mouse IgG. Other band(s): none.