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Catalog Number | orb348278 |
---|---|
Category | Antibodies |
Description | F(ab')2 Mouse IgG (H&L) antibody (Alkaline Phosphatase) |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | IgG F(ab')2 |
Conjugation | AP |
Reactivity | Mouse |
Form/Appearance | Liquid (sterile filtered) |
Concentration | 1.0 mg/mL |
Buffer/Preservatives | 0.1% (w/v) Sodium Azide |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins. |
Immunogen | Anti-Mouse F(ab')2 IgG (H&L) was produced by repeated immunization with Mouse IgG whole molecule in rabbit. |
Tested applications | ELISA, IHC, WB |
Dilution range | ELISA: 1:4,000 - 1:20,000, IHC: 1:200 - 1:1,000, WB: 1:1,000 - 1:5,000 |
Application notes | Anti-Mouse F(ab')2 IgG Alkaline Phosphatase Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency. |
Antibody Type | Secondary Antibody |
Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
Alternative names | Rabbit F(ab')2 Anti-Mouse IgG Antibody alkaline ph Read more... |
Note | For research use only |
ELISA, IHC, WB | |
Mouse | |
Goat | |
Polyclonal | |
AP |
DOT, ELISA, IHC, WB | |
Mouse | |
Goat | |
Polyclonal | |
AP |
ELISA, IHC, WB | |
Mouse | |
Goat | |
Polyclonal | |
AP |
ELISA, IHC, WB | |
Mouse | |
Donkey | |
Polyclonal | |
AP |