Human VEGF165 Protein

• Catalog Number: orb257923
• Category: Proteins
• Description: Human VEGF165, premium grade is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4).
• Target: VEGF165
• Conjugation: Unconjugated
• Tag: Native
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 95%
• UniProt ID: P15692
• MW: 19.2 kDa
• Application notes: This protein carries no "tag". The protein has a calculated MW of 19.2 kDa. The protein migrates as 24 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 0.01 EU per μg
• Expression Region: Ala 27 - Arg 191
• Storage: -20°C to -70°C for 24 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Cytokines
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human VEGF protein

Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations

Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations

Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165, premium grade (Cat. No. orb257923) with an affinity constant of 0.776 nM as determined in a SPR assay (Biacore T200) (Routinely tested).