Human GM-CSF Protein

• Catalog Number: orb257510
• Category: Proteins
• Description: Human GM-CSF, premium grade is expressed from human 293 cells (HEK293). It contains AA Ala 18 - Glu 144 (Accession # NP_000749.2).
• Target: GM-CSF
• Conjugation: Unconjugated
• Tag: Native
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 95%
• UniProt ID: NP_000749.2
• MW: 14.5 kDa
• Application notes: This protein carries no "tag". The protein has a calculated MW of 14.5 kDa. The protein migrates as 25 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 0.01 EU per μg
• Expression Region: Ala 18 - Glu 144
• Storage: -20°C to -70°C for 24 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Cytokines
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human GM-CSF protein

Loaded Human GM-CSF R alpha, Fc Tag (Cat. No. orb545735) on Protein A Biosensor, can bind Human GM-CSF, premium grade (Cat. No. orb257510) with an affinity constant of 9.21 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).