CD31/PECAM1 Mouse Monoclonal Antibody

SKU: orb2956852

Description

Anti-Human CD31/PECAM1 Antibody (BUCC4390) is a mouse monoclonal antibody detecting Platelet endothelial cell adhesion molecule in ELISA, WB, FCM. Suitable for Human. Highlights Multi-Application: Validated across multiple applications. Flow Cytometry: Validated for flow cytometry applications.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, WB
• Reactivity: Human

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: VHH-mFc (IgG2a)
• Clone No.: BUCC4390
• Purity: >95% as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1

Flow-cytometry using anti-human CD31 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD31 monoclonal antibody (Catalog orb2956852, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD31 antibody. THP-1 cells were stained with an irrelevant antibody (Green Histogram) or an anti-human CD31 antibody monoclonal antibody (Catalog # orb2956852, Blue Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human CD31/PECAM1 Antibody.

Quick Database Links

UniProt

P16284