Human Activin A Protein

• Catalog Number: orb16656
• Category: Proteins
• Description: Human Activin A, premium grade is expressed from human 293 cells (HEK293). It contains AA Gly 311 - Ser 426 (Accession # AAH07858.1).
• Target: Activin A
• Conjugation: Unconjugated
• Tag: Native
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: 0.056% TFA in 30% ACN
• Purity: 95%
• UniProt ID: AAH07858.1
• RRID: AB_10749569
• MW: 13.0 kDa
• Application notes: This protein carries no "tag". The protein has a calculated MW of 13.0 kDa. The protein migrates as 14-15 kDa under reducing (R) condition, and 23-25 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 0.1 EU per μg
• Expression Region: Gly 311 - Ser 426
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Cytokines
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

References

Basu, Moitri et al. Invasion of ovarian cancer cells is induced byPITX2-mediated activation of TGF-β and Activin-A Mol Cancer, 14, 162 (2015)

PubMed: 26298390

SDS-PAGE analysis of Human Activin A protein

Immobilized Human Activin A, premium grade (Cat. No. orb16656) at 5 μg/mL (100 μL/well) can bind Human ACVR2A, Fc Tag (Cat. No. orb1101193) with a linear range of 0.4-25 ng/mL (QC tested).