Cytokeratin 6 Rabbit Polyclonal Antibody

SKU: orb182758

Description

Cytokeratin 6 Rabbit Polyclonal Antibody

Research Area

Cancer Research, Epi / Endothelial, Signal Transduction, Tumor Biology, Varscular System

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Cytokeratin 6 (351-450/564aa)
• Target: KRT6A
• Molecular Weight: 60 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

K6E; KRT6E; PPKNEFD; CK-6C; CK-6E; CK6A; CK6C; CK6D; K6A; K6C; K6D; KRT6C; KRT6D; PC3; CK-6B; CK6B; K6B; KRTL1; PC2; PC4; 2310016L08Rik; CK-6A; K6[a]; Krt2-6a; Krt2-6c; MK6a; mK6[a]; Krt2-6b; mK6[b]; K2C6A_HUMAN; KRT6A; Cytokeratin-6A (CK-6A); Cytokeratin-6D (CK-6D); Keratin-6A (K6A); Type-II keratin Kb6; K2C6B_HUMAN; KRT6B; Cytokeratin-6B (CK-6B); Keratin-6B (K6B); Type-II keratin Kb10; K2C6C_HUMAN; Cytokeratin-6C (CK-6C); Cytokeratin-6E (CK-6E); Keratin K6h; Keratin-6C (K6C); Type-II keratin Kb12; K2C6A_MOUSE; Keratin-6-alpha (mK6-alpha); Ker2; Krt2-6; Krt6; K2C6B_MOUSE; Keratin-6-beta (mK6-beta); K6-beta;

Sample: Lane 1: Mouse Skin tissue lysates, Lane 2: Mouse Placenta tissue lysates, Lane 3: Mouse Lung tissue lysates, Primary: Anti-Cytokeratin 6 (orb182758) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kDa, Observed band size: 60 kDa.

Tissue/Cell: Human laryngeal tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Cytokeratin 6 Polyclonal Antibody, Unconjugated (orb182758) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human laryngocarcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CK6B Polyclonal Antibody, Unconjugated (orb182758) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Cytokeratin 6 Polyclonal Antibody, Unconjugated (orb182758) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: mouse stomach tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Cytokeratin 6 Polyclonal Antibody, Unconjugated (orb182758) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

KRT6A

UniProt

P02538