Anti-Cytokeratin-5/6 Antibody

• Catalog Number: orb515473
• Category: Antibodies
• Description: Rabbit polyclonal antibody to KRT5
• Target: KRT5
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytokeratin-5/6. The exact sequence is proprietary.
• UniProt ID: P04259, P48668, P13647, Q922U2, Q6P6Q2, P02538
• Tested applications: IH, WB
• Dilution range: WB: 1:500-1000
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Keratin type II cytoskeletal 5; 58 kDa cytokeratin
• Note: For research use only
• Entrez: 369017, 3852, 110308

Western blot analysis of Cytokeratin-5/6 expression in A375 (A), EC9706 (B), Hela (C), MCF7 (D), PC3 (E), rat ovary (F) whole cell lysates. (Predicted band size: 62; 60 kD; Observed band size: 60 kD)

Immunohistochemical analysis of Cytokeratin-5/6 staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.