CD68 Rabbit Polyclonal Antibody

• Catalog Number: orb10344
• Category: Antibodies
• Description: CD68 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD68 (1-130/354aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 110-140K kDa
• Target: CD68
• UniProt ID: P34810
• RRID: AB_10749240
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CD 68; CD68 antigen; CD68 molecule; DKFZp686M18236
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: A549 (Human) Cell Lysate at 30 ug, Lane 3: Jurkat (Human) Cell Lysate at 30 ug, Lane 4: Huvec (Human) Cell Lysate at 30 ug, Primary: Anti-CD68 (orb10344) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 110/185-200 kD, Observed band size: 110 kD.

Sample: Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug, Lane 2: Lymph node (Rat) Cell Lysate at 30 ug. Primary: Anti-CD68 (orb10344) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 110/185-200 kD, Observed band size: 110 kD.

Paraffin embedded human brain tissue labeled with Rabbit Anti-CD68 Polyclonal Antibody, Unconjugated (orb10344) at 1:50 followed by conjugation to the secondary antibody and Permanent Fast Red Quanto staining.

Tissue/Cell: human mammary cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CD68 Polyclonal Antibody, Unconjugated (orb10344) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.