CD68 antibody

• Catalog Number: orb197999
• Category: Antibodies
• Description: CD68 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Isotype: IgG
• Immunogen: CD68 recombinant protein
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: WB: 2 μg/ml, IHC-P:1:100, IF/ICC: 1:100
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 37 kDa
• Target: CD68
• Entrez: 968
• UniProt ID: P31996, P34810, Q4FZY1
• NCBI: 001040059, 001035148
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti CD68 antibody, anti CD68gen antibody, anti CD
• Note: For research use only
• Application notes: Representative images showing the presence of macrophages in the ovary. Macrophages were identified using marker CD68. Increased expression of macrophage marker CD68 was noted in both the HF-Ln (n¼6) and HF-Ob (n¼6) groups compared to the LF-Ln mice (n¼6), both after 10 (A) and 32 (B) weeks on HFD. These findings indicate increased tissue inflammation in the ovary after prolonged exposure to HFD regardless of obese phenotype. Ctrl, control. Original magnification 3200
• Expiration Date: 12 months from date of receipt.

References

Skaznik-Wikiel, Malgorzata E. et al. High-Fat Diet Causes Subfertility and Compromised Ovarian Function Independent of Obesity in Mice Biol Reprod, 94, 108 (2016)

PubMed: 27030045

Applications

IHC

Reactivity

Mouse

Catarina Barbosa-Matos 1 2, Caroline Borges-Pereira 1 2, Sofia Libório-Ramos 1 2, Raquel Fernandes 1 2, Marcela Oliveira 1 2, Ana Mendes-Frias 1 2, Ricardo Silvestre 1 2, Nuno S Osório 1 2, Hélder N Bastos 3 4 5, Rita F Santos 4 6, Susana Guimarães 7, António Morais 3, Massimiliano Mazzone 8 9, Agostinho Carvalho 1 2, Cristina Cunha 1 2, Sandra Costa Deregulated immune cell recruitment orchestrated by c-MET impairs pulmonary inflammation and fibrosis Respir Res, 25, 257 (2024)

PubMed: 38909206

Applications

IF

Reactivity

Human

Jelodari, Sahar et al. Assessment of the Efficacy of an LL-37-Encapsulated Keratin Hydrogel for the Treatment of Full-Thickness Wounds ACS Appl Bio Mater, (2023)

PubMed: 37224450

Western blot analysis of breast cancer (Lane 1), breast cancer (Lane 2), breast cancer (Lane 3) using CD68 antibody (dilution at 2 ug/ml)

Immunohistochemical staining of rat spleen tissue using CD68 antibody (dilution of primary antibody - 1:100)

IHC-P image of mouse lymph node tissue using anti-CD68 (dilution of primary antibody at 1:100)

IHC-P staining of rat spleen tissue using anti-CD68 (dilution at 1:100)

Representative images showing the presence of macrophages in the ovary. Macrophages were identified using marker CD68. Increased expression of macrophage marker CD68 was noted in both the HF-Ln (n¼6) and HF-Ob (n¼6) groups compared to the LF-Ln mice (n¼6)

Immunofluorescence analysis of mouse lymph node tissue using CD68 antibody (dilution of primary antibody - 1:100)

Representative images showing the presence of macrophages in the ovary. Macrophages were identified using marker CD68. Increased expression of macrophage marker CD68 was noted in both the HF-Ln (n¼6) and HF-Ob (n¼6) groups compared to the LF-Ln mice (n¼6)