CD44 Antibody

SKU: orb69034

Description

Mouse monoclonal antibody to CD44

Images & Validation

• Tested Applications: FC, ICC, IHC, WB
• Dilution range: WB: 1/500-1/1000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Reactivity: Human, Monkey, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 8E2F3
• Immunogen: Purified recombinant fragment of human CD44 (628-699) expressed in E. Coli.
• Target: CD44
• Molecular Weight: 82kDa

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide.
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Disclaimer: For research use only

Alternative Names

IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues with DAB staining using CD44 antibody.

Confocal immunofluorescence analysis of methanol-fixed A431 (A), Hela (B), PANC-1 (C) and EC (D) cells using CD44 antibody

Western blot analysis of truncated Trx-CD44 recombinant protein (lane1) and GST-CD44 recombinant protein (lane2) using CD44 antibody.

Confocal immunofluorescence analysis of PANC-1 cells using CD44 antibody

Western blot analysis of Hela (lane1) and HUVE-12(lane2) cell lysate using CD44 antibody.

Confocal analysis of paraffin-embedded human lung cancer tissues using CD44 antibody

Flow cytometric analysis of Hela cells negative control (left) using CD44 antibody (right)

Quick Database Links

Gene Symbol

CD44

UniProt

P16070

NCBI Gene ID

960

References

Kim, Ryounggo et al. CD44 expression in patients with combined hepatocellular cholangiocarcinoma Ann Surg Treat Res, 89, (2015)

PubMed: 26131439