CD44 Antibody

SKU: orb1294318

Description

Rabbit polyclonal antibody to CD44

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution range: Western Blot 1:1000–1:2000 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1: 100-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human CD44
• Target: CD44

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Anti-CD44 antibody - at 1/1000 dilution, Lysates at 40 µg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294318 overnight at 4°C.

Anti-CD44 antibody - at 1/1000 dilution, Lysates at 40 µg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294318 overnight at 4°C. Vimentin antibody (orb1294389).

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294318 anti-CD44 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294318 anti-CD44 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD44 with orb1294318 at 1/150.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD44 with orb1294318 at 1/150.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD44 with orb1294318 at 1/150.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD44 with orb1294318 at 1/150.

Quick Database Links

Gene Symbol

CD44