CD133 Rabbit Polyclonal Antibody (FITC)

• Catalog Number: orb782916
• Category: Antibodies
• Description: CD133 Rabbit Polyclonal Antibody (FITC)
• Target: PROM1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: FITC
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human CD133 (508-552/865aa)
• Tested applications: FC
• Dilution range: Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: AC133; Antigen AC133; Hematopoietic stem cell anti
• Research Area: Cell Markers, Molecular Biology, Neuroscience, Sur
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: LOVO. Primary Antibody (green line): Rabbit Anti-CD133 antibody (orb782916), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.