Caspase-1 p20 Rabbit Polyclonal Antibody (FITC)

• Catalog Number: orb318090
• Category: Antibodies
• Description: Caspase-1 p20 Rabbit Polyclonal Antibody (FITC)
• Target: CASP1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: FITC
• Reactivity: Human, Mouse, Rat
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from mouse Caspase-1 p20 (181-280/402aa)
• Tested applications: FC, IF
• Dilution range: Flow-Cyt=1μg/Test, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: caspase-1 isoform alpha p20 subunit; CASP1; ICE; I
• Research Area: Apoptosis, Caspases, Cell Cycle, Hypoxia, Metaboli
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Li Xu 1, Jie Su 2, Caili Du 3, Hui Liu 4, Wei Lv Ghrelin suppresses hypoxia/reoxygenation-induced H9C2 cell pyroptosis via NLRP3 Cell Mol Biol (Noisy-le-grand), (2024)

PubMed: 38158667

Applications

IF

Reactivity

Rat

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-CASP1 antibody (orb318090), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-CASP1 antibody (orb318090), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: U937. Primary Antibody (green line): Rabbit Anti-Caspase-1 p20 antibody (orb318090), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.