Caspase-1 p20 Rabbit Polyclonal Antibody

SKU: orb317579

Description

Caspase-1 p20 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse Caspase-1 p20 (181-280/402aa)
• Target: CASP1
• Molecular Weight: 20/46 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

caspase-1 isoform alpha p20 subunit; CASP1; ICE; IL1BC; Caspase-1 subunit p20; CASP 1; Caspase 1 apoptosis related cysteine peptidase; Caspase 1 apoptosis related cysteine protease; Caspase1; IL 1 beta converting enzyme; IL 1BC; IL1B convertase; IL1BCE; Interleukin 1 beta convertase; Interleukin 1 beta convertase precursor; Interleukin 1 beta converting enzyme; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45; CASP1_HUMAN; CASP1_MOUSE.

Blank control (blue line): MCF7 (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice), Primary Antibody (green line): Rabbit Anti-Caspase-1 p20 antibody (orb317579), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC.

Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-Caspase-1 p20 antibody (orb317579), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: large intestine (rat) Lysate at 40 ug, Primary: Anti-Caspase-1 p20 (orb317579) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20/46kD, Observed band size: 46kD.

Sample: lung (rat) Lysate at 40 ug, Primary: Anti-Caspase-1 p20 (orb317579) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20/46kD, Observed band size: 46kD.

Tissue/Cell: Mouse spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Caspase-1 p20 Polyclonal Antibody, Unconjugated (orb317579) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

CASP1

UniProt

P29452