ALOX15 Rabbit Polyclonal Antibody

• Catalog Number: orb101113
• Category: Antibodies
• Description: ALOX15 Rabbit Polyclonal Antibody
• Target: ALOX15
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Rat
• Predicted Reactivity: Bovine, Human, Mouse, Porcine
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from mouse ALOX15/15 Lipoxygenase 1 (581-662/662aa)
• MW: 73 kDa
• Tested applications: WB
• Dilution range: WB=1:500-2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 15 lipoxygenase 1; 15 LIPOXYGENASE RETICULOCYTE AR
• Research Area: Cancer Research, Cardiovascular Research, Cell Bio
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-15-LOX Polyclonal Antibody, Unconjugated (orb101113) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.