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Item 1 of 4
ALOX15 Antibody (C-term)
Catalog Number: orb1928784
| Catalog Number | orb1928784 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This ALOX15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 610-643 amino acids from the C-terminal region of human ALOX15. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| UniProt ID | P16050 |
| MW | 74804 Da |
| Tested applications | IF, IHC-P, WB |
| Dilution range | IF: 1:10~50, WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50 |
| Antibody Type | Primary Antibody |
| Clone Number | RB17156 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Arachidonate 15-lipoxygenase, 15-LOX, 15-LOX-1, 12 Read more... |
| Note | For research use only |
| NCBI | NP_001131.3 |
Confocal immunofluorescent analysis of ALOX15 Antibody (C-term) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
ALOX15 Antibody (C-term) western blot analysis in Hela (lane 1), A549 (lane 2), K562 (lane 3), MDA-MB435 (lane 4) cell line lysates (35 ug/lane).This demonstrates the ALOX15 antibody detected the ALOX15 protein (arrow).
Formalin-fixed and paraffin-embedded human lymph tissue reacted with ALOX15 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes: Anti-ALOX15 Antibody (C-term) at 1:2000 dilution. Lane 1: K562 whole cell lysate. Lane 2: MDA-MB-453 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 75 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
