AIF Rabbit Polyclonal Antibody

SKU: orb1294405

Description

Rabbit polyclonal antibody to Iba1

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: Western Blot 1:500-1:1000, Immunofluorescence 1:100-1:300, Immunohistochemistry (Paraffin) 1:100-1:300, Immunohistochemistry (Frozen) 1:100-1:300
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Target: AIF / Iba1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Disclaimer: For research use only

Frozen sectioned for immunohistochemical analysis. Sample: Frozen sectioned Rat brain. Green: Iba1 antibody (orb1294405), Red: TUJ1 antibody, Blue: DAPI.

Iba1/AIF1 antibody - at 1/500 dilution, Lysates at 45 µg per lane, This blot was produced using a 15% SDS-PAGE. PVDF membrane was then blocked with 3% BSA for an hour before being incubated with orb1294405 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded Mouse brain tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded Rat brain tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical of frozen sections. Sample: mouse cerebellum. Green: Iba1 antibody (orb1294405): 1-200, Anti-rabbit 488: 1-500. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical of frozen sections. Sample: mouse hippocampus. Green: Iba1 antibody (orb1294405): 1-200, Anti-rabbit 488: 1-500. DAPI (blue) was used as the nuclear counter stain.

Quick Database Links

Gene Symbol

AIF / Iba1