5-Ethynyl-2'-deoxyuridine (5-EdU)

SKU: orb1734893

Description

5-EdU (5-Ethynyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-EdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymi-dine. The resulting ethynyl-functionalized DNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging.

Images & Validation

• Application Notes:
  DNA synthesis monitoring. ; Spectroscopic Propertie: max 288 nm, 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• CAS Number: 61135-33-9
• MW: Theoretical MW: 252.23 g/mol; Detected MW: 252.07 g/mol
• Purity: 98% (HPLC)
• Formula: C₁₁H₁₂N₂O₅
• SMILES: n1(cc(c(=O)[nH]c1=O)C#C)[C@H]1C[C@@H]([C@H](O1)CO)O

Storage & Handling

• Storage: store at -20 °C. store dry and under inert gas. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Disclaimer: For research use only